The effects of polyamines on the cyclic AMP efflux and metabolism in E. coli B cells |
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| Authors: | G Illiano G F Draetta A Laurenza A Spina G Paolisso |
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| Affiliation: | 1. Department of Orthopedics, Shuang Ho Hospital, Taipei Medical University, Taipei 23561, Taiwan;2. School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei 11031, Taiwan;3. Research Center of Geriatric Nutrition, College of Nutrition, Taipei Medical University, Taipei 11031, Taiwan;4. Department of Medical Research & Education, Taipei Medical University - Shuang Ho Hospital, New Taipei City 235, Taiwan;5. Department of Urology, Taipei Medical University - Shuang Ho Hospital, New Taipei City 235, Taiwan;6. Department of Pathology, Taipei Medical University-Shuang Ho Hospital, New Taipei City, Taiwan;7. Department of Biotechnology and Pharmaceutical, Yuanpei University of Medical Technology, No. 306, Yuanpei Street, Hsinchu, Taiwan;8. School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei City 110, Taiwan;9. Department of Dentistry, Taipei Medical University - Shuang Ho Hospital, New Taipei City 235, Taiwan;10. Department of Medical Laboratory Science and Biotechnology, Yuanpei University of Medical Technology, Hsinchu City 30015, Taiwan |
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| Abstract: | - 1.1. The effects of polyamines spermine, spermidine and putrescine have been investigated on the adenylate cyclase and phosphodiesterase activities bound to E. coli B-cell membranes.
- 2.2. Putrescine, which is physiologically present in bacteria, affects the cAMP metabolism in a coordinate manner, depressing the adenylate cyclase and stimulating the phosphodiesterase activities. In vivo this is reflected by a decreased cAMP efflux toward the external medium.
- 3.3. Spermidine stimulates the phosphodiesterase activities but has no effect on the adenylate cyclase; furthermore no effect was observed in vivo on the cAMP efflux. li[4. Spermine stimulates both the enzymic activities but with a prevailing effect on the adenylate cyclase. In vivo the effect is an increase in the cAMP efflux.
- 4.5. In vivo the intracellular cAMP content is anyway not modified in presence of polyamines, in contrast with (and may be because of) the broad variation of the nucleotide efflux toward the external medium.
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