Detection of simian immunodeficiency virus DNA in macrophages from infected rhesus macaques. |
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| Authors: | R E Unger M L Marthas A A Lackner E Pratt-Lowe B L Lohman K Van Rompay P A Luciw |
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| Institution: | Department of Medical Pathology, University of California, Davis. |
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| Abstract: | We have examined the frequency of infection of monocyte-derived and alveolar macrophages isolated from rhesus macaques inoculated with simian immunodeficiency virus (SIVmac) utilizing a semiquantitative PCR methodology. Animals were inoculated with either pathogenic (SIVmac239) or nonpathogenic (SIVmac1A11) molecularly cloned viruses of SIVmac, or with uncloned pathogenic SIVmacBIOL. The frequency of SIV DNA in macrophages was highest early after infection and at terminal stages of disease, whereas during the asymptomatic period, SIV DNA was present at very low levels in macrophages. |
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| Keywords: | polymerase chain reaction SIVmac1A11 SIVmac239 frequency of infection monocyte-derived macrophages alveolar macrophages |
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