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Sequence of the gene encoding the 16S rRNA of the beer spoilage organismMegasphaera cerevisiae
Authors:L M Doyle  J O McInerney  J Mooney  R Powell  A Haikara  Dr A P Moran
Affiliation:(1) Biotechnology and Food Research Laboratory, Technical Research Centre of Finland, Espoo, Finland;(2) Department of Microbiology, University College, University Road, Galway, Ireland
Abstract:The 16S ribosomal RNA gene from the beer-spoilage organism,Megasphaera cerevisiae was polymerase chain reaction (PCR)-amplified and sequenced. Analysis confirmed the phylogenetic position ofM. cerevisiae as a sister taxon ofMegasphaera elsdenii, within the obligately anaerobic, Gram-negative cocci. The sequence obtained should facilitate the development of DNA probes for early detection of this spoilage organism.
Keywords:Megasphaera cerevisiae  16S rRNA  DNA probe  beer spoilage  phylogeny
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