Growth and amino acid requirements of hyaluronic-acid-producing Streptococcus zooepidemicus |
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| Authors: | D C Armstrong M J Cooney M R Johns |
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| Institution: | (1) Department of Chemical Engineering, The University of Queensland, Brisbane Qld 4072, Australia. Fax: 61 7 3365 4199, AU |
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| Abstract: | A chemically defined medium has been developed for anaerobic cultivation of hyaluronic-acid(HA)-producing Streptococcus zooepidemicus, which contains 11 amino acids essential to growth, and glutamine as a principal nitrogen source. The final HA concentration,
the specific rate of HA production and HA-to-glucose yields were similar for growth in the chemically defined medium relative
to growth in complex medium. Consequently cells cultivated on chemically defined medium can be expected to have similar activity
regarding HA synthesis as compared to cells grown on complex media. However, the specific growth rate and volumetric HA production
rate were found to be less favourable in the chemically defined media.
Received: 4 October 1996 / Accepted: 25 October 1996 |
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