Detection and resolution of genetic loci affecting circadian period in <Emphasis Type="Italic">Brassica oleracea</Emphasis> |
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Authors: | Neeraj Salathia James R Lynn Andrew J Millar Graham J King |
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Institution: | (1) FAS Center for Systems Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA;(2) Warwick HRI, Wellesbourne, Warwick, CV35 9EF, UK;(3) University of Edinburgh, King’s Building, Edinburgh, EH9 3JH, UK;(4) Rothamsted Research, Harpenden, AL5 2JQ, UK |
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Abstract: | Circadian rhythms regulate many aspects of plant growth, fitness and vigour. The components and detailed mechanism of circadian
regulation to date have been dissected in the reference species Arabidopsis thaliana. To determine the genetic basis and range of natural allelic variation for intrinsic circadian period in the closest crop
relatives, we used an accurate and high throughput data capture system to record rhythmic cotyledon movement in two immortal
segregating populations of Brassica oleracea, derived from parent lines representing different crop types. Periods varied between 24.4 and 26.1 h between the parent lines,
with transgressive segregation between extreme recombinant lines in both populations of ∼3.5 h. The additive effect of individual
QTL identified in each population varied from 0.17 to 0.36 h. QTL detected in one doubled haploid population were verified
and the mapping intervals further resolved by determining circadian period in genomic substitution lines derived from the
parental lines. Comparative genomic analysis based on collinearity between Brassica and Arabidopsis also allowed identification of candidate orthologous genes known to regulate period in Arabidopsis, that may account for the additive circadian effects of specific QTL. The distinct QTL positions detected in the two populations,
and the extent of transgressive segregation suggest that there is likely to be considerable scope for modulating the range
of available circadian periods in natural populations and crop species of Brassica. This may provide adaptive advantage for optimising growth and development in different latitudes, seasons or climate conditions. |
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