秦岭地区大林姬鼠的酯酶和苹果酸脱氢酶同工酶的研究

用聚丙烯酰胺凝胶等电聚焦电泳分析了大林姬鼠(Apodemus peninsulae)心、肝、脾、肾和腿肌的 α-酯酶，β-酯酶和苹果酸脱氢酶同工酶。结果表明3种同工酶的活性在5种器官组织中均有明显差异，其中以肝组织的酯酶活性最高，不同器官组织的酶谱也有明显差别，如脾的β一酯酶仅有B区带，同一器官组织通常以α-酯酶活性高于β-酯酶。苹果酸脱氢酶在碱性溶液中染色，肝组织有明显的AB医。心肌与腿肌的苹果酸脱氢酶活性略高于其他组织。

STUDIES ON THE ESTERASE AND MALATE DEHYDROGENASE ISOZYM E ZYM 0GRAM S 0F APODEMUS PENINSULAE OF THE REGION QIN LING

The thin—layer isoelectric focusing electrophoresis with polyacrylamide gel method wereused to analyse the α-esterase(α-est),β-esterase(β-est)and Malate dehydrogcnase(M DH ) isozymes in the five kinds of tissues of Apodemus peninsulae．The sampies covered heart，liver，spleen，kidney and muscle．The results show that all the three kinds of isozyme have obvious tissue specificity．There are clear different enzyme activities in the five tissues．The Esterase activity in liver is higher than in other four tissues．The zymograms in the different issues have distinct differences as well. Spleenis different from other tissues，its β—Est only existing in region and band B．In the same tissue，the α-Est and β-Est are different too．Normally，the activity of α-Est ishigher than ofβ-Eat．The MDH of liver has an evident region AB when it is stained in alkaline solution,but spleen has none．The MDH activities of heart and muscle area little higher than of other tissues．