Electroinduced release of invertase from Saccharomyces cerevisiae |
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| Authors: | V. Ganeva B. Galutzov J. Teissie |
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| Affiliation: | (1) Department of Biophysics and Radiobiology, Sofia University, 8 blvd Dragan Tzankov, 1164 Sofia, Bulgaria;(2) IPBS, UMR 5089-CNRS, 205 Route de Narbonne, 31077 Toulouse, France |
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| Abstract: | Invertase liberation from Saccharomyces cerevisiae was detected after application of series of rectangular millisecond electric pulses. Maximal yield (60% from the activity in crude extract) was achieved within 8 h after pulsation. As shown by staining SDS-PAGE for invertase activity, the main part of liberated enzyme is a high molecular weight periplasmic invertase. |
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| Keywords: | electropulsation extraction invertase Saccharomyces cerevisiae |
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