Puromycin enhances 20-hydroxyecdysone-induced protein synthesis in wing discs of Drosophila melanogaster |
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Authors: | Frank Y T Sin |
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Institution: | (1) Department of Zoology, University of Canterbury, Christchurch 1, New Zealand |
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Abstract: | The effect of cycloheximide and puromycin on 20-hydroxyecdysone-induced protein synthesis in wing discs of Drosophila melanogaster has been studied by one-dimensional and two-dimensional SDS polyacrylamide electrophoresis. It is found that puromycin, but not cycloheximide, when applied simultaneously with the hormone enhanced the hormone-induced synthesis of the early and late proteins. However, when puromycin was applied after hormone treatment, only the late proteins were induced. The possible implication of these observations is discussed. |
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Keywords: | Drosophila 20-hydroxyecdysone puromycin protein synthesis cycloheximide imaginal wing disc |
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