Analysis of parotid and mixed saliva in Roe deer (Capreolus capreolus L.) |
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Authors: | J Fickel F Göritz B A Joest T Hildebrandt R R Hofmann G Breves |
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Institution: | Department of Evolutionary Genetics, Institute for Zoo Biology and Wildlife Research, Alfred-Kowalke-Stra?e 17, D-10315 Berlin, Germany Tel.: ++49 30 516 8726, Fax: ++49 30 512 6104, e-mail: fickel@IZW-berlin.de, DE Department of Physiology, School of Veterinary Medicine, Bischofsholer Damm 15/102, D-30173 Hannover, Germany, DE
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Abstract: | In ruminants, different functions have been ascribed to the different salivary glands according to the feeding type. In this
context, possible adaptations of salivary functions were investigated regarding the secretion of various proteins by different
types of salivary glands. To yield uncontaminated parotid saliva in large quantities, a non-surgical method has been developed.
Parotid gland secretions were collected via endoscopic placement of guide wires into each parotid duct, which were subsequently
used for placement of collection catheters. Salivary flow was stimulated by intra-glandular administration of the parasympathomimetic
compound pilocarpine-hydrochloride into the parotid gland. Mixed saliva (excluding parotid saliva) was collected into sterile
tubes by normal outflow during the sampling of parotid saliva. The total flow volume, flow rate and the content of proteins
as well as of several ions (Na+, K+, Ca2+, inorganic phosphate) of both types of saliva were measured in sheep, fallow deer and roe deer. Roe deer secreted the highest
amount of total salivary proteins relative to body mass mg/kg body mass] and the highest relative volume ml/10 min/kg body
mass], both in parotid and mixed saliva, of all ruminant species examined. Additionally, the protein profile and the tannin-binding
properties of parotid and mixed saliva in roe deer were investigated. Parotid saliva bound almost twice as much tannin as
mixed saliva, underlining the importance of yielding uncontaminated parotid saliva for tannin-binding studies.
Accepted: 6 January 1998 |
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Keywords: | Roe deer Salivary proteins Tannin-binding |
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