Selection of microorganisms which produce raw-starch degrading enzymes |
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Authors: | Frederico W Bergmann Jun-ichi Abe Susumu Hizukuri |
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Institution: | (1) Department of Agricultural Chemistry, Kagoshima University, 21-24, Korimoto-1, 890 Kagoshima, Japan |
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Abstract: | Summary Microorganisms which produce strong raw-starch degrading enzymes were isolated from soil using a medium containing a unique carbon source, -amylase resistant starch (-RS), which is insoluble in water and hardly digested with Bacillus amyloliquefaciens -amylase. Among the isolates, three strains showing high activities were characterized. Two of them, K-27 (fungus) and K-28 (yeast), produced -amylase and glucoamylase, and the final product from starch was only glucose. The third strain, K-2, was a bacterium and produced -amylase, which produced glucose and malto-oligosaccharides from starch. The enzyme preparation of these strains degraded raw corn starch rapidly. |
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