Biodegradation of diazo dye Direct brown MR by Acinetobacter calcoaceticus NCIM 2890 |
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| Authors: | Gajanan Ghodake Sheetal Jadhav Vishal Dawkar Sanjay Govindwar |
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| Institution: | 1. Department of Biochemistry, Shivaji University, Vidyanagar, Kolhapur 416 004, Maharashtra, India;2. Department of Microbiology, Shivaji University, Vidyanagar, Kolhapur 416 004, Maharashtra, India;1. Department of Biotechnology Engineering, NMAM Institute of Technology, Nitte, Karnataka, India;2. Department of Chemistry, and Centre for Energy Science, Indian Institute of Science Education and Research, Pashan, Pune, 411008, India;3. Department of Biotechnology, Bioscience Division, Kuvempu University, Shankaraghatta, Shimoga, Karnataka, India;1. Department of Environmental Science and Engineering, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, 200234, PR China;2. School of Environment, Tsinghua University, Beijing 100084, PR China;1. Department of Agriculture and Biotechnology, Wenzhou Vocational College of Science and Technology, 325006 Wenzhou, Zhejiang, PR China;2. College of Life Science, Zhejiang University, 310058 Hangzhou, Zhejiang Province, PR China;1. Department of Natural Resources and Environmental Engineering, Hanyang University, Hangdang dong Sungdong Gu, Seoul 133-791, South Korea;2. Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur 416004, MS, India;3. Department of Biochemistry, Shivaji University, Vidyanagar, Kolhapur 416004, MS, India;4. Department of Applied Life Chemistry, Institute of Agriculture and Life Sciences, Gyeongsang National University, 900 Gajwa-dong, Jinju 660-071, South Korea |
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| Abstract: | Acinetobacter calcoaceticus was employed for the degradation of Direct brown MR (DBMR), commercially used azo dye in the textile industry in order to analyze mechanism of the degradation and role of inhibitors, redox mediators and stabilizers of lignin peroxidase during decolorization. Induction of intracellular and extracellular lignin peroxidase, intracellular laccase and DCIP reductase represented their involvement in the biodegradation of DBMR. Decolorization and biodegradation of azo dye DBMR in broth were monitored by UV–visible spectrophotometer and TLC. The products obtained from A. calcoaceticus degradation were characterized by FTIR and identified by GC/MS as biphenyl amine, biphenyl, 3-amino 6-hydroxybenzoic acid and naphthalene diazonium. Germination (%) and growth efficiency of Sorghum vulgare and Phaseolus mungo seeds revealed the degradation of DBMR into less toxic products than original dye. A. calcoaceticus also has a potential to degrade diverse dyes present in the textile effluent, into nontoxic metabolites, hence A. calcoaceticus can be applied for the commercial application. |
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