Efficient expression and characterization of hepatitis B virus preS2 antigen in Escherichia coli |
| |
Authors: | Young Jun Kang Young Shin Kim Hyangsuk Hur Hee Sun Kim Hyo Jeong Hong |
| |
Institution: | (1) The Antibody Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology, KIST, P.O. Box 115, Yuseong, Taejon , 305-600, Korea |
| |
Abstract: | The complete (encoding 55 amino acids, aa) or partial (encoding aa 1–26) preS2 region gene of hepatitis B virus (HBV) was fused to the 3-end of glutathion-S-transferase (GST) gene and expressed under the control of the inducible tac promoter in Escherichia coli at 37 °C. The fusion protein with the complete preS2 region was moderately expressed (8%) while the protein with the N-terminal 26 aa was expressed at a higher level, yielding about 20% of the total cellular proteins. The GST-preS2 (aa 1–26) protein, which contains the immunodominant epitope, was produced form the soluble protein fraction of the recombinant bacteria and purified by affinity chromatography using glutathione-agarose column. The purified preS2 fusion protein showed the antigenicity of preS2, as assessed by indirect and competitive ELISAs. |
| |
Keywords: | Escherichia coli expression hepatitis B virus preS2 antigen |
本文献已被 SpringerLink 等数据库收录! |
|