|
|||||
|
|
| 锌指核酸酶技术的应用 | |
| 引用本文： | 张伟锋,夏海滨. 锌指核酸酶技术的应用[J]. 生命的化学, 2011, 0(1) |
| 作者姓名： | 张伟锋 夏海滨 |
| 作者单位： | 陕西师范大学基因治疗研究室; |
| 基金项目： | 农业部转基因生物新品种培育科技重大专项子课题(2009ZX08010-023B)资助 |
| 摘 要： | 锌指核酸酶(zinc finger nuclease,ZFN)技术是近年来发展起来的一种对基因组DNA实现靶向修饰的新技术。ZFN通过作用于基因组DNA上特异的靶位点产生DNA双链切口(double strand break,DSB),然后经过非同源末端连接(non-homologous end joining,NHEJ)或同源重组(homologous recombination,HR)途径实现对基因组DNA的靶向敲除或者替换。该技术近些年来已经被广泛应用于基因靶向修饰的研究。本文在简要介绍ZFN技术的基础上,重点综述了目前该技术在基因靶向修饰中的应用研究进展,并同时对该技术目前所需解决的一些问题以及未来的研究方向进行了分析。 |
| 关 键 词： | 锌指核酸酶 靶向修饰 同源重组 基因治疗 |
Application of zinc finger nuclease |
|
| Abstract: | Zinc finger Nuclease (ZFN) technique is recently developed for targeted modification of genome DNA. The double strand break induced by ZFN is repaired through non-homologous end joining (NHEJ) or homologous recombination (HR) pathway through which gene knockout or replacement will be generated. It has been widely used in gene-targeted modification these years. Based on a brief introduction of ZFN, we mainly focus on the application of zinc finger nuclease in gene-targeted modification in animals and plants.... |
| Keywords: | zinc finger nuclease targeting modification homologous recombination gene therapy |
| 本文献已被 CNKI 等数据库收录！ | |