Measurement of Electron Transport System (ETS) activity in soil |
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| Authors: | J. T. Trevors C. I. Mayfield W. E. Inniss |
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| Affiliation: | (1) Department of Biology, University of Waterloo, N2L 3G1 Waterloo, Ontario, Canada |
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| Abstract: | Electron transport system (ETS) activity was measured in amended and nonamended soil by measuring the reduction of 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride (INT) to iodonitrotetrazolium formazan (INT-formazan), which can be easily extracted with methanol without interference from other compounds found in soil. A high correlation between ETS activity and oxygen consumption was observed. This technique allows rapid quantitative measurements of microbial ETS activity in soil. |
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