Effects of cadmium accumulation on growth and respiration of a cadmium-sensitive strain of Bacillus subtilis and a selected cadmium resistant mutant |
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Authors: | Surowitz Kenneth G. Titus Jeffrey A. Pfister Robert M. |
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Affiliation: | (1) Department of Microbiology, The Ohio State University, 43210 Columbus, OH, USA;(2) Present address: Bristol Myers Company, 13221-4755 Syracuse, NY, USA |
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Abstract: | The effects of cadmium on the growth and respiration of two strains of Bacillus subtilis are compared to the accumulation of Cd by viable and cyanide-killed cells, protoplasts and cell fractions of the strains. Growth and respiration of strain 1A1 were significantly inhibited at 10g Cd2+/ml while the growth and respiration of strain 1A1R, a selected mutant of 1A1, were only slightly affected. Similarly, 1A1R protoplasts were more resistant to Cd than were 1A1 protoplasts. The differential resistance of the strains correlates with the accumulation of Cd by the two strains, with 1A1 accumulating approximately 10 times the level of Cd after a 4 h exposure to 1 g Cd2+/ml. The distributions of Cd throughout the cells, however, were similar between strains. Based on the accumulation of Cd by cyanide-killed protoplasts, uptake of Cd by 1A1 appears to be an active process, while for 1A1R, Cd accumulation is independent of protoplast viability.Non-standard abbreviations SMM Subtilis Minimal Medium - AAS Atomic Absorption Spectrophotometry - TSA Trypticase Soy Agar - PCA Plate Count Agar - INT 2-p-iodophenyl-3-p-nitrophenyl-5-phenyl-2H tetrazolium chloride - dd H2O double distilled demineralized water - OD Optical Density |
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Keywords: | Cadmium Toxicity Accumulation Bacillus subtilis |
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