Assignment of the human gamma-glutamyl transferase gene to the long arm of chromosome 22 |
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Authors: | F Bulle M G Mattei S Siegrist A Pawlak E Passage M N Chobert Y Laperche G Guellaën |
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Institution: | (1) Institut National de la Santé et de la Recherche Médicale, U-99, Hôpital H. Mondor, F-84010 Créteil, France;(2) Institut National de la Santé et de la Recherche Médicale, U-242, Hôpital des Enfants de la Timone, F-13385 Marseille Cedex 5, France |
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Abstract: | Summary We have determined the chromosomal location of the human gene for gamma-glutamyltransferase (GGT). This study was done by in situ hybridization of human metaphase spreads with a rat cDNA probe specific for this enzyme and constructed from two clones previously characterized in our laboratory. The final construct had a 1.6-kb-long insert covering 92% of the coding sequence for GGT. The new insert was also freed of any GC tails introduced for the cDNA cloning, because we observed that these sequences were responsible for a high background. Using this probe for the analysis of 136 human metaphase spreads, we observed a strong specific signal on chromosome 22 at the interface of q111-112 and a minor peak in q131. Thus GGT might represent a new marker for the study of certain diseases which have chromosomal abnormalities at these loci. |
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