Molecular identification and genotyping of Blastocystis sp. in sheep and goats from some areas in Inner Mongolia,Northern China |
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| Institution: | 1. College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, PR China;2. International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450046, PR China;3. Key Laboratory of Quality and Safety Control of Poultry Products (Zhengzhou), Ministry of Agriculture and Rural Affairs, Zhengzhou 450046, PR China;4. Norman Bethune Health Science Center of Jilin University, Changchun 130021, Jinlin Province, PR China;5. College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China;1. Laboratory of Parasitology, Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, Campus Vida, 15782 Santiago de Compostela, A Coruña, Spain;2. Nanotechnology and Integrated BioEngineering Centre, School of Engineering, Ulster University, Belfast Campus, 2-24 York Street, Belfast BT15 1AP, United Kingdom;3. Doñana Biological Station (EBD-CSIC), Avenida Américo Vespucio s/n 41001, Sevilla, Spain;4. Research Institute on Chemical and Biological Analysis, University of Santiago de Compostela, 15782 Santiago de Compostela, A Coruña, Spain;1. School of Science, Mae Fah Luang University, Chiang Rai, Thailand;2. Gut Microbiome Research Group, Mae Fah Luang University, Chiang Rai, Thailand;3. Laboratory of Molecular and Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Canterbury, United Kingdom |
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| Abstract: | Blastocystis sp. is a kind of unicellular intestinal commensal which is widely distributed in humans and animals, and frequently found in the people who are in close contact with animals. To investigate the prevalence and evaluate the zoonotic potential of Blastocystis sp. in sheep and goats from Inner Mongolia, China, a total of 1037 samples were collected from them, and subjected to nested PCR amplification based on the small subunit ribosomal RNA (SSU rRNA) gene of Blastocystis sp. The sanger sequencing was used for Blastocystis sp. subtype identification. The results indicated that the average infection rate of Blastocystis sp. was 10.70% 95CI: 8.82%–12.58%] (111/1037), including 11.30% 95CI: 7.96%–14.64%] for sheep (39/345) and 10.40% 95CI: 8.13%–12.67%] for goats (72/692). Five Blastocystis subtypes (ST5, ST10, ST14, ST21 and ST26) were identified in the present study. Among them, ST10 was the most dominant subtype in sheep and goats, accounting for 70.27% (78/111) of the total identified positive samples. This is the first report regarding Blastocystis sp. subtypes ST21 and ST26 in goats in China. This study has provided a detail epidemiological data on the prevalence and subtypes distribution of Blastocystis sp. in sheep and goats in Inner Mongolia, China. Our results indicated that sheep and goats could be reservoir host for multiple Bastocystis subtypes, including the zoonotic subtypes. Further studies among humans, livestock and wild animals are needed to better understand their role in the spread of Blastocystis sp. |
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