Leishmania amazonensis DNA in wild females of Lutzomyia
cruzi (Diptera: Psychodidae) in the state of Mato Grosso do Sul,
Brazil |
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Authors: | Everton Falc?o de Oliveira Aline Etelvina Casaril Nathália Lopes Fontoura Mateus Paula Guerra Murat Wagner Souza Fernandes Elisa Teruya Oshiro Alessandra Gutierrez de Oliveira Eunice Aparecida Bianchi Galati |
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Institution: | 1.Universidade de São Paulo, Faculdade de Saúde Pública, Programa de Pós-Graduação em Saúde Pública, São Paulo, SP, Brasil;2.Universidade Federal de Mato Grosso do Sul, Centro de Ciências Biológicas e da Saúde, Campo Grande, MS, Brasil;3.Universidade de São Paulo, Faculdade de Saúde Pública, Departamento de Epidemiologia, São Paulo, SP, Brasil |
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Abstract: | Studies on natural infection by Leishmania spp of sandflies
collected in endemic and nonendemic areas can provide important information on the
distribution and intensity of the transmission of these parasites. This study sought
to investigate the natural infection by Leishmaniain wild female
sandflies. The specimens were caught in the city of Corumbá, state of Mato Grosso do
Sul (Brazil) between October 2012-March 2014, and dissected to investigate
flagellates and/or submitted to molecular analysis to detect
Leishmania DNA. A total of 1,164 females (77.56% of which were
Lutzomyia cruzi) representing 11 species were investigated using
molecular analysis; 126 specimens of Lu. cruziwere dissected and
also submitted to molecular analysis. The infection rate based on the presence of
Leishmania DNA considering all the sandfly species analysed was
0.69%; only Leishmania (Leishmania) amazonensis was identified in
Lu. cruzi by the molecular analysis. The dissections were
negative for flagellates. This is the first record of the presence of L. (L.)
amazonensis DNA in Lu. cruzi, and the first record of
this parasite in this area. These findings point to the need for further
investigation into the possible role of this sandfly as vector of this parasite. |
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Keywords: | natural infection Lutzomyia cruzi Leishmania amazonensis molecular biology polymerase chain reaction dissection |
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