A possible circulation of a dominant Dibothriocephalus nihonkaiensis haplotype in Japan revealed by molecular analysis of clinical tapeworm samples |
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| Institution: | 1. Department of Parasitology and Tropical Medicine, Tokyo Medical & Dental University, Tokyo, Japan;2. Department of Parasitology and Tropical Medicine, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan;3. Department of Parasitology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Accra, Ghana;4. Department of Molecular Parasitology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan;1. Department of Medical Zoology, Mie University School of Medicine, Mie, Tsu 514-8507, Japan;2. Department of Molecular Protozoology, Research Center for Infectious Disease Control, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan;1. Department of Parasitology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh;2. Department of Parasitology and Tropical Medicine, Kitasato University School of Medicine, Minami, Sagamihara, Kanagawa, Japan;3. Department of Parasitology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon, Accra, Ghana;1. Medical Parasitology Department, Faulty of Medicine, Tanta University, Egypt;2. Biochemistry Division, Chemistry Department, Faculty of Science, Damanhour University, Egypt;3. Zoology Department, Faculty of Science, Tanta University, Egypt;4. Clinical Pathology Department, Faculty of Medicine, Tanta University, Egypt;5. Anatomy and Embryology Department, Faculty of Medicine, Tanta University, Egypt;6. Histopathology Department, Faculty of Medicine, Tanta University, Egypt;7. Physiology Department, Faculty of Medicine, Tanta University, Egypt;8. Biostatistics and Public Health Department, Faculty of Medicine, Menoufia University, Egypt;9. Medical Parasitology Subunit, Microbiology and Immunology Department, Faculty of Medicine, Mutah University, Jordan;10. Medical Parasitology Subunit, Pathology Department, College of Medicine, Jouf University, Sakaka, Saudi Arabia |
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| Abstract: | Human diphyllobothriasis, caused by Dibothriocephalus nihonkaiensis, is prevalent globally, especially in regions where raw fish is consumed. Recent molecular diagnostic techniques have made species identification of tapeworm parasites and the determination of genetic variations among parasite populations possible. However, only a few studies done over a decade ago, have reported on the genetic variation among D. nihonkaiensis in Japan. The present study employed PCR-based mitochondrial DNA analysis to specifically detect D. nihonkaiensis from archived clinical samples, and to determine any genetic variation that may exist among the Japanese broad tapeworms from patients of Kanagawa Prefecture, Japan. Target genes were amplified from DNA extracted from the ethanol- or formaldehyde-fixed samples by PCR. Further sequencing and comparative phylogenetic analyses based on mitochondrial COI and ND1 sequences were also performed. In our results, all PCR-amplified and sequenced samples were identified as D. nihonkaiensis. Analysis of COI sequences revealed two haplotype lineages. However, clustering of almost all COI (and ND1) sample sequences into one of the two haplotype clades, together with reference sequences from different countries worldwide, revealed a common haplotype among D. nihonkaiensis samples in our study. Our results suggest a possible presence of a dominant D. nihonkaiensis haplotype, with a global distribution circulating in Japan. Results from this study have the potential to improve the management of clinical cases and establish robust control measures to reduce the burden of human diphyllobothriasis in Japan. |
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