Short-term storage sperm of coho salmon (Oncorhynchus kisutch) at 4 °C: Effect of sperm: Extender dilution ratios and antioxidant butyl-hydroxytoluene (BHT) on sperm function |
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| Institution: | 1. Southern Scientific Center, Russian Academy of Sciences, st. Chehova, 41, Rostov-on-Don, 344006, Russia;2. Astrakhan State Technical University, ul. Tatishcheva 16, Astrakhan, 414025, Russia;3. North Ossetian State University, Vladikavkaz, 362040, Russia;4. Lomonosov Moscow State University, Leninskie gory 1/3, Moscow, 119991, Russia;1. Tunceli University, Fisheries Faculty, 62000 Tunceli, Turkey;2. Muğla Sıtkı Koçman University, Faculty of Fisheries, 48000 Muğla, Turkey;3. Aksaray University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Aksaray, Turkey;4. Aksaray Vocational School, Aksaray University, Aksaray, Turkey;1. Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Departamento de Aquicultura, Laboratório de Piscicultura Marinha, Florianópolis, SC, CEP 88062-601, Brazil;2. Núcleo de Investigación en Producción Alimentaria, Escuela de Acuicultura, Universidad Católica de Temuco, Temuco, Chile;3. Departamento de Ingeniería Química, Facultad de Ingeniería y Ciencias, Universidad de La Frontera, Temuco, Chile;4. BIOREN – Center for Biotechnology in Reproduction, La Frontera University, Temuco, Chile;5. Biochemical and Pharmaceutical Technology Department, School of Pharmaceutical Sciences, University of São Paulo, Av. Lineu Prestes 580, São Paulo, 05508-900, Brazil;1. Center of Biotechnology on Reproduction (BIOREN‐CEBIOR), Faculty of Medicine, Universidad de La Frontera, Temuco, Chile;2. Department of Preclinical Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile;3. Department of Basic Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile;1. University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zatisi 728/II, 38925 Vodnany, Czech Republic;2. Sino-Czech Joint Laboratory for Fish Conservation and Biotechnology, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, China;3. University of South Bohemia in Ceske Budejovice, Faculty of Science, Institute of Chemistry and Biochemistry, Branisovska 1760, 37005 Ceske Budejovice, Czech Republic;4. Biology Centre of Academy of Sciences of the Czech Republic, Institute of Parasitology, Branisovska 31, 37005 Ceske Budejovice, Czech Republic;5. CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao 266101, China |
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| Abstract: | Short-term storage of semen is a necessary key procedure in fish; it allows maximizing the use of gametes. Nevertheless, sperm quality decreases during storage has been associated with oxidative stress damage due to an increase in reactive oxygen species (ROS) during storage. This study was designed to optimize a short-term storage protocol for Coho salmon (Oncorhynchus kisutch) spermatozoa, evaluating the effect of extender dilution and the addition of butyl-hydroxytoluene (BHT) antioxidant on sperm function parameters. In the first experiment, fresh semen was diluted in Storfish®: extender dilution (1:2 and 1:3) and a control sample undiluted and stored at 4 °C for 7-days. In both experiments motility (MO), viability and integrity of plasma membrane, mitochondrial membrane potential (MMP) and superoxide anion level (O2−) were evaluated at 0, 3 and 7 days. Result shows that, 1:3 dilution maintained a higher sperm function for a longer period time. In the second experiment, spermatozoa were suspended in Storfish® (1:3) supplemented with two different concentrations of BHT (1.0 mM and 2.0 mM) and a control sample without antioxidant and stored at 4 °C for 7 days. The results demonstrated that, antioxidant-supplemented samples greater MO than control samples (P < 0.05). The viability remained >75% during storage in all groups. MMP was higher in 2.0 mM BHT compared to 1.0 mM and control (P < 0.05), in addition, this concentration reduced O2− level (P < 0.05). In conclusion, sperm: extender dilution 1:3 and adding of 2.0 mM BHT in sperm storage extender may enhance protection sperm function in Oncorhynchus kisutch against effects harmful of the oxidative stress during the in vitro storage. |
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| Keywords: | Sperm Short-term storage Sperm: extender dilution Butylated hydroxytoluene Oxidative stress |
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