Elamipretide as a potential candidate for relieving cryodamage to human spermatozoa during cryopreservation |
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| Institution: | 1. Swine research center, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, SP, Brazil;2. Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu, São Paulo, Brazil;1. School of Health & Sports Science and School of Nursing, School of Viticulture, Eastern Institute of Technology, Taradale Campus, Hawkes Bay, 4112, New Zealand;2. Andrology Laboratory, Hammersmith Hospital, Imperial College NHS Trust, London, W120HS2, United Kingdom;1. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran;2. Urology and Nephrology Research Center (UNRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran;1. Division of Animal Reproduction, ICAR-Indian Veterinary Research Institute, Bareilly, 243122, Uttar Pradesh, India;2. BEMI Section, ICAR-Indian Veterinary Research Institute, Bareilly, 243122, Uttar Pradesh, India;3. Division of Veterinary Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Bareilly, 243122, Uttar Pradesh, India |
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| Abstract: | As a technique widely used in assisted reproduction, human spermatozoa cryopreservation makes it possible to conserve functional sperm for a long time, but the impact of cryodamage on sperm during the process could not be ignored. The objective of the present study was to investigate the efficacy of Elamipretide, a novel small mitochondrial targeting short cytoprotective peptide, in attenuating cryodamage during spermatozoa cryopreservation. Semen samples were collected and cryopreserved in freeze solution containing different concentrations (0.0, 0.1, 1, and 10 μM) of Elamipretide. Sperm motility, viability, membrane integrity, mitochondrial membrane potential, DNA fragmentation, antioxidant profiles, and acrosome reaction were measured and analyzed. The results showed that supplementation of the freeze media with Elamipretide (1 and 10 μM) significantly improved post-thaw sperm parameters including motility and viability, stability of the plasma membrane, and mitochondria and chromosomes. In addition, by adding Elamipretide, excessive oxidation and acrosome dysfunction in sperm cells undergoing freeze-thaw were also significantly attenuated. Therefore, Elamipretide may be a potential candidate for relieving cryodamage to human spermatozoa during cryopreservation. |
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| Keywords: | Cryopreservation Elamipretide Sperm Mitochondria |
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