Characterization of 42 polymorphic microsatellite loci in Mimulus ringens (Phrymaceae) using Illumina sequencing |
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| Authors: | Schyler O Nunziata Jeffrey D Karron Randall J Mitchell Stacey L Lance Kenneth L Jones Dorset W Trapnell |
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| Institution: | 1. Savannah River Ecology Laboratory, University of Georgia, Aiken, South Carolina 29802 USA;2. Department of Biological Sciences, P. O. Box 413, University of Wisconsin–Milwaukee, Milwaukee, Wisconsin 53201 USA;3. Department of Biology, University of Akron, Akron, Ohio 44325 USA;4. University of Colorado School of Medicine, 12801 E. 17th Ave., Aurora, Colorado 80045 USA;5. Department of Plant Biology, University of Georgia, Athens, Georgia 30602 USA |
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| Abstract: | ? Premise of the study: Microsatellite markers were isolated and characterized in Mimulus ringens (Phrymaceae), a herbaceous wetland perennial, to facilitate studies of mating patterns and population genetic structure. ? Methods and Results: A total of 42 polymorphic loci were identified from a sample of 24 individuals from a single population in Ohio, USA. The number of alleles per locus ranged from two to nine, and median observed heterozygosity was 0.435. ? Conclusions: This large number of polymorphic loci will enable researchers to quantify male fitness, patterns of multiple paternity, selfing, and biparental inbreeding in large natural populations of this species. These markers will also permit detailed study of fine-scale patterns of genetic structure. |
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| Keywords: | Illumina paired-end microsatellite Mimulus ringens PAL-finder PCR primers simple sequence repeat markers |
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