Cross-linked agarose for separation of low molecular weight natural products in hydrophilic interaction liquid chromatography |
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| Authors: | Tianwei Tan Zhi-Guo Su Ming Gu Jun Xu Jan-Christer Janson Professor |
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| Institution: | 1. Department of Bioengineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China;2. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China;3. Department of Physical and Analytical Chemistry, Uppsala Biomedical Center, Uppsala University, Uppsala, Sweden |
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| Abstract: | Following its market introduction in 1982, the cross-linked 12% agarose gel media Superose 12 has become widely known as a tool for size exclusion chromatography of proteins and other biological macromolecules. In this review it is shown that, when appropriate mobile phases are used, Superose possesses adsorption properties similar to that of traditional media for hydrophilic interaction liquid chromatography (HILIC). This is illustrated by the separation and purification of low molecular weight compounds such as polyphenols including active components of traditional Chinese medicinal herbs and green tea. Structural features of the cross-linked agarose that likely cause the observed adsorption effects are discussed aswell. These are identified as being primarily ether bonds acting as strong hydrogen bond acceptors as well as hydrophobic residues originating from the cross-linking reagents. |
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| Keywords: | Cross-linked agarose Green tea catechin HILIC Mixed-mode separation Polyphenol separation |
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