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Expression patterns and association analysis of the porcine DHX58 gene |
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| Authors: | X Y Li C M Han Y Wang H Z Liu Z F Wu Q H Gao S H Zhao |
| Institution: | 1. Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
These authors contributed equally to this work.;2. Animal Science Technology, Talim University, Alar, Xinjiang 843300, China. These authors contributed equally to this work.;3. Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, Hubei, China.;4. College of Animal Science, Huanan Agricultural University, Guangzhou, 510642, Guangdong, China;5. Animal Science Technology, Talim University, Alar, Xinjiang 843300, China. |
| Abstract: | RIG-1 signalling is responsible for the detection of cytoplasmic viral RNA molecules. DEXH (Asp-Glu-X-His) box polypeptide 58 (encoded by DHX58) is a negative regulator of the RIG-1 signalling pathway. In human, the DHX58 gene can be upregulated and can inhibit the RIG-1 signalling pathway during viral infection. In this study, porcine DHX58 gene expression patterns were studied. According to our results, the porcine DHX58 gene was upregulated not only by the stimulation of Poly I:C but also by the stimulation of 1ipopolysaccharides (LPS). One polymorphism (g.4919G>C), detected in the ninth intron, was significantly associated with some blood parameters including the red cell distribution width of 1-day-old pigs and white blood cell counts, lymphocyte absolute counts, and platelet distribution width of 17-day-old pigs (P < 0.05). Moreover, the individuals with the genotype GG have a significantly higher mean white blood cell count than individuals with genotype CC or GC (P < 0.05). Our study indicates that DHX58 is an important gene that is associated with the immune response in swine. |
| Keywords: | association analysis DHX58 expression pig polymorphism |