Enzyme activity of the phenylpropanoid pathway as a response to apple scab infection |
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| Authors: | A Slatnar M Mikulic Petkovsek H Halbwirth F Stampar K Stich R Veberic |
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| Institution: | 1. University of Ljubljana, Biotechnical Faculty, Agronomy Department, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia;2. Technical University of Vienna, Institute for Chemical Engineering, Getreidemarkt 9/1665, A-1060 Vienna, Austria |
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| Abstract: | The study was performed on apple trees, ‘Golden Delicious' cv., which is a scab-susceptible cultivar. The phenolic content of apple fruit was determined in different parts of the peel. The phenolic compounds were analysed in the scab spot, in the tissue around the spot and in the healthy tissue. We determined the concentration of various phenolic compounds and related enzyme activities. Infection with the Venturia inaequalis fungus enhanced the metabolism of phenolic compounds at the scab spot, around the spot and in healthy peel. Compared with the healthy tissue and the tissue around the spot, the scab spot showed higher enzyme activity for all tested enzymes, except for dihydrochalcone 2′-O-glucosyltransferase, which had lower activity in the scab spot. In comparison to the healthy peel, the scab spot showed up to 3.4 times more hydroxycinnamic acids, up to 1.1 times more dihydrochalcones and up to 1.4 times more flavan-3-ols. In contrast, the healthy peel showed up to 1.6 times more flavonols than the scab spot. |
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| Keywords: | Dihydrochalcone 2′-O-glucosyltransferase flavonoids fruits Malus domestica phenolic compounds phenylpropanoid pathway phenylalanine ammonia lyase Venturia inaequalis |
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