Production,purification and characterisation of a novel halostable xylanase from Bacillus sp. NTU-06 |
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| Authors: | C-Y Wang H Chan H-T Lin Y-T Shyu |
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| Institution: | 1. Department of Horticulture, National Taiwan University, Taipei, Taiwan;2. Section of Molecular and Cellular Biology, University of California, Davis, CA, USA;3. Department of Food Science, Nutrition and Nutraceutical Biotechnology, Shih Chien University, Taipei, Taiwan |
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| Abstract: | Bacillus sp. NTU-06 was used to produce xylanase, which is an important industrial enzyme used in the pulp and paper industry. The enzyme was purified by fast protein liquid chromatography (FPLC) and had a molecular mass of 24 kDa. The enzyme was active over a concentration range of 0–20% sodium chloride in culture broth, although its activity was optimal in 5% sodium chloride. A salinity stability test showed that 43% of the enzyme activity was retained after 4 h in 20% sodium chloride. Xylanase activity was maximal at pH 8.0 and 40°C. The enzyme was somewhat thermostable, retaining 20% of the original activity after incubation at 70°C for 4 h. The xylanase had Km and Vmax values of 3.45 mg mL−1 and 387.3 µmol min−1mg−1, respectively. The deduced internal amino acid sequence of Bacillus sp. NTU-06 xylanase resembled the sequence of beta-1,4-endoxylanase, which is a member of glycoside hydrolase family 11. Some of the novel characteristics that make this enzyme potentially effective in xylan biodegradation are discussed. |
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| Keywords: | Bacillus sp halostable purified xylanase |
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