Unusual phosphatase activity resistant to SDS and pronase treatments in Xenopus ovary. |
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Authors: | T Sugimoto M Amano T Tokumoto K Ishikawa |
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Institution: | Department of Biology and Geosciences, Faculty of Science, Shizuoka University, Ohya 836, Shizuoka, 422-8529, Japan. |
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Abstract: | An unusual phosphatase, which is resistant to treatment by 5% SDS and proteolytic enzymes, was isolated as two types, 1 and 2, from pronase-treated homogenates of Xenopus ovary. The molecular sizes of types 1 and 2 were estimated as about 140 kDa and more than 2 x 10(4) kDa, respectively, by gel filtration, but as 140 and 130 kDa as a catalytic unit, respectively, by electrophoresis, implying that whereas type 1 might be composed of catalytic unit alone, type 2 is a multicomponent complex consisting of a 130-kDa catalytic unit. Both activities were sensitive to nucleases but resistant to tested proteolytic enzymes. These findings suggest that the unusual phosphatase activity is attributable to a polynucleotide. |
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