Temporal and spatial variations of lipid droplets during adipocyte division and differentiation |
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Authors: | Nagayama Masafumi Uchida Tsutomu Gohara Kazutoshi |
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Institution: | Division of Applied Physics, Graduate School of Engineering, Hokkaido University, North 13, West 8, Sapporo 060-8628, Japan. macci@eng.hokudai.ac.jp |
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Abstract: | By capturing time-lapse images of primary stromal-vascular cells (SVCs) derived from rat mesenteric adipose tissue, we revealed temporal and spatial variations of lipid droplets (LDs) in individual SVCs during adipocyte differentiation. Numerous small LDs (a few micrometers in diameter) appeared in the perinuclear region at an early stage of differentiation; subsequently, several LDs grew to more than 10 microm in diameter and occupied the cytoplasm. We have developed a method for the fluorescence staining of LDs in living adipocytes. Time-lapse observation of the stained cells at higher magnification showed that nascent LDs (several 100 nm in diameter) grew into small LDs while moving from lamellipodia to the perinuclear region. We also found that adipocytes are capable of division and that they evenly distribute the LDs between two daughter cells. Immunofluorescence observations of LD-associated proteins revealed that such cell divisions of SVCs occurred even after LDs were coated with perilipin, suggesting that the "final" cell division during adipocyte differentiation occurs considerably later than that characterized in 3T3-L1 cells. Our time-lapse observations have provided a detailed account of the morphological changes that SVCs undergo during adipocyte division and differentiation. |
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Keywords: | time-lapse observation fluorescence observation live cell imaging visceral adipocyte adipogenesis adipose conversion lipid bodies lipid droplet-associated proteins |
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