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Co-dominant markers for the selection of somatic dihybrids and polyhybrids
Authors:L Celotti  D Furlan  G Lanfranchi  A Sartori  G Marin
Institution:Istituto di Biologia Animale, Universitá di Padova, I-35100 Padova, Italy
Abstract:Resistance to ouabain and puromycin are shown to represent very useful co-dominant characters for the selection of somatic hybrids between mammalian cells, after fusion with polyethylene glycol. We therefore used, with success, a number of Chinese hamster and mouse cell lines carrying these markers in association with thymidine kinase and hypoxanthine-guanine-phosphoribosyl transferase deficiency for selection of hybrids of triparental origin.
Keywords:To whom offprint requests should be addressed  
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