非洲钝缘蜱感染Q热立克次体敏感度的测定

Q热立克次体以10^-1—10^-12不同稀释度,用血腔注射法感染非洲钝缘蜱,感染35天,取蜱血淋巴液,分别用Gimenez染色法及免疫荧光技术检查,在10^-1—10^-5的稀释度,两种方法检出率基本相似。10^-6以下各组,免疫荧光尚可检出少数阳性标本,而Gimcnez法则无阳性,前者总阳性率为48.28%,后者为40.68%。感染的蜱悬液接种豚鼠,12组动物血清做Q热补体结合试验全部阳性。动物发病情况如潜伏期长短、发烧天数等似与蜱内立克次体的含量有关。

THE SUSCEPTIBILITY OF ORNITHODOROS MOUBATA TO THE MICROORGANISM COXIELLA BURNETJ

Ornithodoros moubata were expermentally infected with Coxiella burneti through the haemocoel. The dilutions of the infected chick yolk sacs were prepared in series of 10-fold from 10-1-10-12. Each of these dilutions was inoculated into each group of. 5 ticks. The haemolymph samples from these ticks were taken on the 35th day after inoculation. In the haematocyte test, the microorganisms in the body were examined both with Gimenez stain and immunofluoreseence technique.The results of the experiments showed that in large-doses such as 10-1-10-5, the microorganisms in the haemolymph samples were found similar by these two techniques. However, in lower-doses as 10-7, 10-8 and 10-10, they were positive by immunofluorescence technique, but negative by Gimenez stain.The positive percentage in haematocyte test with Gimenez stain was 40.68% and by immunofluorescence technique was 48.28%. Therefore, the immunofluorescence technique is more sensitive than the Gimenez stain in the detection of C. burneti in haematocyte test.12 groups of guinea pigs were inoculated with the dilutions of the suspension of infected ticks for 48 days, the blood samples for complement-fixation test were taken by cardiac puncture on the 26th day after inoculation. All of the guinea pigs inoculated were complement-fixation test positive, but the incubation period and the duration of fever among these guinea pigs suffered from C. burneti infections varied in these groups of different dilutions: The length of incubation period was 1 day (on average) in groups of dilutions of 10-1-10-3; 2.6 days (on average) in dilutions of 10-4-10-6, and extended to 4.6 days (on average) in dilutions of 10-7-10-12. The duration of fever were for 5 days (on average) in groups of dilutions of 10-1-10-3; 4.6 days (on average) in dilutions of 10-4- 10-6, and shorten to two days (on average) in the other dilutions of 10-7-10-12.It is suggested that the incubation period and durations of fever among the infected guinea pigs are in corrlation with the amount of C. burneti in the body of the infected O. moubata.