|
|||||
|
|
| 过量表达水杨酸羟基酶NahG降低烟草对青枯菌的抗性 | |
| 引用本文： | 党峰峰,林金辉,陈成聪,陈永萍,官德义,何水林.过量表达水杨酸羟基酶NahG降低烟草对青枯菌的抗性[J].广西植物,2013,33(2):177-180. |
| 作者姓名： | 党峰峰 林金辉 陈成聪 陈永萍 官德义 何水林 |
| 作者单位： | 1. 福建农林大学,生命科学学院,福州,35002 2. 福建农林大学,园艺学院,福州,35002 3. 福建农林大学,作物遗传改良和综合利用教育部重点实验室,福州,35002 4. 福建农林大学,生命科学学院,福州,35002;福建农林大学,作物遗传改良和综合利用教育部重点实验室,福州,35002 |
| 基金项目： | 国家自然科学基金(项目编号：30971718),国家高等教育博士研究基因重大专项(项目编号：20093535110004)转基因重大专项(项目编号：2009ZX08001-015B)福建省自然科学基金(项目编号：2008J049) |
| 摘 要： | 研究构建了pMDC32-NahG植物表达载体,并获得了其超表达的转基因烟草T1代株系。结果表明:这些株系与其野生型植株没有明显表型差异,但却表现出较野生型植株更敏感的青枯菌侵染抗性。同时,研究还发现NahG的超表达还显著提高了茉莉酸(JA)-依赖的基因NtPR1-b的表达,降低了(SA)-依赖的相关基因NtPR3和NtPRQ的表达。这表明SA累积的缺陷降低了烟草对青枯菌侵染的抗性。 |
| 关 键 词： | 水杨酸 NahG 青枯菌 |
Overexpression of salicylate hydroxylase NahG declines resistance to Ralstonia solanacearum infection in tobacco |
|
| DANG Feng-Feng,LIN Jin-Hui,CHEN Cheng-Cong,CHEN Yong-Ping,GUAN De-Yi,HE Shui-Lin.Overexpression of salicylate hydroxylase NahG declines resistance to Ralstonia solanacearum infection in tobacco[J].Guihaia,2013,33(2):177-180. | |
| Authors: | DANG Feng-Feng LIN Jin-Hui CHEN Cheng-Cong CHEN Yong-Ping GUAN De-Yi HE Shui-Lin |
| Institution: | 1.College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2.National Education Minster Key laboratory of Crop Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 3.College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
| Abstract: | Studies showed that the plant expression vector of pMDC32-NahG was construed in tobacco K326,and obtained NahG-OE T1 lines. Comparing to wild type K326,NahG-OE lines did not exhibited any phenotypic difference,but exhibited higher susceptibility to Ralstonia solanacearum inoculation. Meanwhile,it was found to be coupled with up-regulated expression of JA-depended NtPR1-b,and down-regulated expression of SA-depended NtPR3 and NtPRQ. Results suggested that SA-degrading NahG tobacco plants declined resistance to R.solanacearum infection. |
| Keywords: | salicylic acid NahG Ralstonia solanacearum |
| 本文献已被 万方数据 等数据库收录！ | |
| 点击此处可从《广西植物》浏览原始摘要信息 | |
| 点击此处可从《广西植物》下载免费的PDF全文 | |