Long-Term Cultivation of Primmorphs from Freshwater Baikal Sponges <Emphasis Type="Italic">Lubomirskia baikalensis</Emphasis> |
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Authors: | Lubov I Chernogor Natalia N Denikina Sergey I Belikov Alexander V Ereskovsky |
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Institution: | 1.Laboratory of Analytical Bioorganic Chemistry,Limnological Institute of the Siberian Branch of Russian Academy of Sciences,Irkutsk,Russia;2.Department of Embryology, Faculty of Biology and Soils,Saint-Petersburg State University,St. Petersburg,Russia;3.Centre d’Océanologie de Marseille, Station marine d’Endoume - CNRS UMR 6540-DIMAR,Marseille,France |
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Abstract: | The work was aimed at performing long-term cultivation of primmorphs in vitro from freshwater sponge Lubomirskia baikalensis (Pallas 1776), collected from Lake Baikal, obtaining its long-term primmorph culture in both natural (NBW) and artificial
(ABW) Baikal water and at identifying the impact of different environmental factors on formation and growth of primmorphs.
The first fine aggregates of L. baikalensis are formed in vitro 10–15 min after dissociation of sponge cells. Epithelization of aggregates begins 4 h later after the
dissociation. Young primmorphs are formed 1 or 2 days later. The surface of primmorphs is covered with a layer of exopinacocytes.
The primmorphs remain viable for more than 10 months at 3–6°C. Over 50% of primmorphs in NBW and 25% in ABW are attached to
the substrate and grow like adult sponges. Thus, the long-term primmorph cultivation in vitro allows the creation of a controlled
live model system under experimental conditions. The results of this work will allow the creation of a cell culture collection
of Baikal freshwater sponges for studying morphogenesis of primmorphs during cultivation at different stages and transdifferentiation
of their cells, physiological functions of sponge cells, processes of spiculogenesis, identification of proteins involved
in biomineralization process, decoding of their genes, as well as a spectrum of secondary metabolites. |
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