Efficacy of tendon stem cells in fibroblast-derived matrix for tendon tissue engineering |
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| Affiliation: | 1. Program in Molecular and Cellular Biology, University of Washington, Seattle, Washington, USA;2. Department of Neurology, University of Washington, Seattle, Washington, USA;3. Department of Biochemistry, University of Washington, Seattle, Washington, USA;4. Department of Medicine, University of Washington, Seattle, Washington, USA;5. Current address: Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan, USA;1. Department of Virology, Pasteur Institute of Iran, Tehran, Iran;2. School of Advanced Technologies in Medicine, Department of Medical Biotechnology, Tehran University of Medical Sciences, Tehran, Iran;3. Endocrinology and Metabolic Research Institute of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran;4. Medical Genetics Department, Tehran University of Medical Sciences, Tehran, Iran;5. Department of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Tehran University of Medical Sciences, Tehran, Iran;6. National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran;1. Division of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata, Japan;2. Division of Oral and Maxillofacial Surgery, Institute of Medicine and Dentistry, Niigata University, Niigata, Japan;3. Division of Periodontology, Institute of Medicine and Dentistry, Niigata University, Niigata, Japan;4. Midwest Biomedical Research Foundation, Research Service, Department of Veterans Affairs Medical Center, Kansas City, Missouri, USA;5. Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, Niigata, Japan;1. Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia;2. Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Lembah Pantai, Kuala Lumpur, Malaysia;3. Department of Orthopedics & Traumatology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia |
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| Abstract: | Background aimsAfter injury, tendons often heal with poor tissue quality and inferior mechanical properties. Tissue engineering using tendon stem cells (TSCs) is a promising approach in the repair of injured tendon. Tenogenic differentiation of TSCs needs an appropriate environment. More recently, the acellular extracellular matrix (ECM) generated from fibroblasts has been used to construct various engineering tissues. In this study, we successfully developed an engineered tendon tissue formed by seeding TSCs in de-cellularized fibroblast-derived matrix (dFM).MethodsPatellar TSCs and dermal fibroblast were isolated and cultured. Using the method of osmotic shock, dFM was obtained from dermal fibroblast. ECM proteins in dFM were examined. TSCs at passage 3 were seeded in dFM for 1 week. Proliferative capacity and characterization of TSCs cultured in dFM were determined by population doubling time, immunofluorescence staining and quantitative reverse transcriptase polymerase chain reaction. Engineered tendon tissue was prepared with dFM and TSCs. Its potentials for neo-tendon formation and promoting tendon healing were investigated.ResultsdFM is suitable for growth and tenogenic differentiation of TSCs in vitro. Neo-tendon tissue was formed with tendon-specific protein expression when TSCs were implanted together with dFM. In a patellar tendon injury model, implantation of engineered tendon tissue significantly improved the histologic and mechanical properties of injured tendon.ConclusionsThe findings obtained from our study provide a basis for potential use of engineered tendon tissue containing dFM and TSCs in tendon repair and regeneration. |
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| Keywords: | differentiation extracellular matrix repair tendon stem cells |
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