Effects of human adipose-derived stem cells on the viability of rabbit random pattern flaps |
| |
| Affiliation: | 1. Department of Plastic and Reconstructive Surgery, Shanghai 9th People''s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People''s Republic of China;2. Biologics and Bioprocess Department, Wuxi AppTec BioPharmaceuticals Co Ltd, Shanghai, P.R. China;3. Department of Plastic Surgery, 1st Affiliated Hospital of Bengbu Medical College, Anhui, People''s Republic of China;1. State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, The Fourth Military Medical University, No. 145 West Changle Road, Xi''an 710032, China;2. Department of Stomatology, The 463 Hospital of People''s Liberation Army, No. 46 XiaoHeYan Road, Shenyang 110042, China;3. State Key Laboratory of Military Stomatology, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, No. 145 West Changle Road, Xi''an 710032, China;4. State Key Laboratory of Military Stomatology, Department of Oral Biology, School of Stomatology, The Fourth Military Medical University, No. 145 West Changle Road, Xi''an 710032, China;1. State Key Laboratory of Genetic Engineering, Department of Pharmaceutical Sciences, School of Life Sciences, Fudan University, Shanghai 200433, China;2. Center of Burns and Traumatic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;1. Immune Intervention, Cell Biology & Immunology Group, Wageningen University, Wageningen, The Netherlands;2. Epitopoietic Research Corporation, Namur, Belgium;3. INSERM U970, PARCC, Université Paris Descartes, Paris, France;4. Service d''Immunologie Biologique, Hopital Européen Georges Pompidou, Paris, France;5. Advanced Cell Immunotherapy Unit, Department of Pharmacology, Medical Faculty, Masaryk University, Brno, Czech Republic;6. Cellthera Ltd, Brno, Czech Republic;7. Department of Neurosurgery, Les Cliniques du Sud Luxembourg, Arlon, Belgium;8. Department of Immunology, Center of Oncosurgery, Institute of Oncology, Vilnius University, Vilnius, Lithuania;1. Department of Bioengineering and Institute for Biotechnology and Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal;2. Centro Hospitalar de Lisboa Ocidental, E.P.E, Hospital São Francisco Xavier, Lisboa, Portugal |
| |
| Abstract: | Background aimsFlap necrosis is the most commonly encountered outcome influencing the effect of operations in clinical practice. The advent of cytotherapy and regenerative medicine with stem cells, especially adipose-derived stem cell therapy, appears to be a promising approach in providing multi-lineage differentiating cells. However, autologous stem cells are limited in both quantity and quality in aging individuals. Hence, xenogenic stem cell therapy was used in this study.MethodsRandom pattern flaps (6 cm × 2 cm) were prepared in a rabbit model transplanted either with 4 × 105 human adipose-derived stem cells at five sites or equal volumes of Dulbecco's modified Eagle's medium. At 7 days after operation, the viability of the flaps from both groups was evaluated. We determined the numbers of locally infiltrating T cells, whereas the CD4/CD8 ratio, interferon, interleukin (IL)-2, IL-4 and IL-10 in the serum were determined to evaluate the immunological response of the rabbit. Moreover, Dil labeling was administrated to trace the homing of the transplanted stem cells.ResultsBoth the survival areas and the capillary number of the flaps that were injected with human adipose-derived stem cells significantly increased as compared with the control group (P < 0.05). Additionally, no significant difference in the immune response was detected between the groups. Dil-labeled stem cells were found to participate in the formation of tubular structures, which were further shown to be CD31+, although not predominantly.ConclusionsHuman adipose-derived stem cells could be used therapeutically to improve the viability of random pattern flaps without detection of serious immune rejection of stem cells. |
| |
| Keywords: | adipose-derived stem cells immunomodulatory random pattern flap vascularization xenogenic transplantation |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
