Increased Inactivation of Messengers in <Emphasis Type="Italic">Escherichia coli ts</Emphasis> Mutant |
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Authors: | MICHEL SIMON DAVID APIRION |
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Institution: | 1.Department of Microbiology,Washington University School of Medicine,St Louis |
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Abstract: | WE recently described some of the properties of a temperature sensitive mutant of Escherichia coli (refs. 1–3 and unpublished work) in which RNAase II activity is increased on transfer to the non-permissive temperature1,2, while the functional half-life of β-galactosidase mRNA1 and the chemical half-life of the lac Operon mRNA3 are decreased. Questions raised by these studies were (a) can the strain be considered a general messenger RNAase mutant and (b) what is the direction of messenger inactivation in this strain? The latter question is particularly interesting since the increased RNAase activity in this strain is that of RNAase II (unpublished work) which degrades RNA molecules in the 3′ to 5′ direction4, while mRNA is known to be degraded in the 5′ to 3′ direction5,6. |
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