Cytokinin-binding proteins |
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Authors: | M Brault R Maldiney E Miginiac |
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Institution: | Laboratoire de Physiologie du Dévelopement des Plantes URA 2135 CNRS. Caster 156. Univ. Pierre et Marie Curie, 4 place Jussieu, F-75252 Paris Cedex 05. France. |
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Abstract: | This article is focused on the modalities of reception of cytokinins which remain largely unknown. It summarizes the main steps of the different protocols used to study cytokinin-binding proteins (CBPs). We place emphasis on the significance and specificity of the detection according to the properties of the probes used: radioactive or photoreactive cytokinins, fluorescent anticytokinins, anti-idiotype antibodies. The purification procedures are also examined. The cellular localisation and the putative physiological roles of the numerous and different CBPs found are considered. The interest of genetic and molecular studies is discussed. |
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Keywords: | Binding protein cytokinin mutant plant growth regulator receptor |
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