| 马铃薯纺锤形块茎类病毒基因的化学合成与克隆 |
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| 引用本文： | 郭蜀光,张婕.马铃薯纺锤形块茎类病毒基因的化学合成与克隆[J].病毒学报,1991,7(2):180-182. |
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| 作者姓名： | 郭蜀光 张婕 |
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| 作者单位： | 河南大学生物系，复旦大学遗传所，复旦大学遗传所，中国医学科学院医学生物研究所，复旦大学遗传所，复旦大学遗传所 上海，上海，上海，上海 |
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| 摘 要： |
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| 关 键 词： | PSTV 化学合成基因 克隆 |
CHEMICAL SYNTHESIS AND CLONING OF A GENE FOR POTATO SPINDLE TUBER VIROID |
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| Guo Shuguang Zhang Jie Min Yongjie Peng Xiaozhong Xie Yi Wang Qisong.CHEMICAL SYNTHESIS AND CLONING OF A GENE FOR POTATO SPINDLE TUBER VIROID[J].Chinese Journal of Virology,1991,7(2):180-182. |
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| Authors: | Guo Shuguang Zhang Jie Min Yongjie Peng Xiaozhong Xie Yi Wang Qisong |
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| Abstract: | potato spindle tuber viroid ( PSTV ) is a plant viroid. It contains 359 nucleotides. The whole of gene was divided into three parts. Those parts contain the respective structural gene of 173bp, 163bp and l8bp.The gene fragment was chemically synthesized by DNA synthesizer. The synthetic gene was inserted into plasmid M13mp18, and transformed into JM109 strain. The clones containing the anticipated gene sequence was obtained by hybridization. The accuracy of sequence was confirmed by DNA sequencing. |
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| Keywords: | Potato spindle tuber viroid Chemical synthesis |
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