The in vitro effect of a thymic epithelial culture supernatant on mixed lymphocyte reactivity and intracellular cAMP levels of thymocytes and on antibody production to SRBC by Nu/Nu spleen cells. |
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| Authors: | A M Kruisbeek G C Astaldi M J Blankwater J J Zijlstra L A Levert A Astaldi |
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| Institution: | 1. Institute for Experimental Gerontology, REPGO-TNO, 151 Lange Kleiweg, Rijswijk, The Netherlands;2. Central Laboratory of the Netherlands Red Cross Blood Transfusion Service and the Laboratory of Experimental and Clinical Immunology of the University of Amsterdam, Amsterdam, The Netherlands |
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| Abstract: | Addition of supernatants from rat thymic epithelial cultures (TES) to rat thymocytes stimulated with T-cell-mitogens or allogeneic cells leads to an increase in 14C-TdR incorporation. Furthermore, in the presence of TES, spleen cells from athymic nu/nu mice exhibit an enhanced in vitro antibody production to SRBC, whereas TES has no such effect on spleen cells from T-cell-deprived mice. If TES is added together with thymocytes to T-cell-deprived spleen cell cultures, the number of plaque-forming cells to SRBC is enhanced, suggesting that TES induces a helper cell function in thymocytes which, if added alone, have no effect. TES also increases intracellular levels of cAMP in thymocytes in vitro and appears to act on a membrane site distinct from the β-adrenergic receptor. TES fails to affect mitogen responses, MLR and cAMP levels of lymphocytes from other lymphoid organs. The biological activity of TES as compared to that of thymic extracts is discussed. |
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