Cultivation of transgenicNicotiana tabacum suspension cells in bioreactors for the production of mGM-CSF |
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| Authors: | Sang-Yoon Lee Won Hur Gyu Heon Cho Dong-Il Kim |
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| Institution: | (1) Department of Biological Engineering and Center of Advanced Bioseparation Technology, Inha University, 402-751 Incheon, Korea;(2) Department of Biotechnology, Kangwon National University, 200-701 Chunchon, Korea;(3) Department of Chemical Engineering, Kangwon National University, 200-701 Chunchon, Korea |
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| Abstract: | TransgenicNicotiana tabacum cells were cultivated for the production of murine granulocyte macrophage-colony stimulating factor (mGM-CSF) in both a stirred,
tank biore|actor and an airlift bioreactor with draft tube. Cell growth and mGM-CSF production in the airlift bioreactor were
found to be better than those achieved in the stirred tank bioreactor. In the airlift bioreactor. 9.0 g/L of cells and 2.2
ng/mL of mGM-CSF were obtained (11.0 g/L and 2.4 ng/mL, respectively in shake flasks). Although the lag period was prolonged
and mGM-CSF production was lowered by 33% in the stirred tank bioreactor as compared to the control culture, the maximum cell
density was increased up to 12.0 g/L due to better mixing by agitation at the higher cell density. |
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| Keywords: | bioreactor foreign protein mGM-CSF plant cell culture transgenic plant |
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