Fluorescence in situ hybridization for direct visualization of Gram-negative anaerobes in subgingival plaque samples |
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| Authors: | H. Gersdorf,K. Pelz,U.B. Gö bel |
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| Affiliation: | Institut für Medizinische Mikrobiologie und Hygiene, Klinkum der Albert-Ludwigs-Universität, Freiburg, FRG |
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| Abstract: | Abstract Fluorescent oligonucleotide probes complementary to variable regions of Porphyromonas gingivalis and Bacteroides forsythus 16S ribosomal RNA were used to identify these organisms in smears of formaldehyde-fixed subgingival plaque samples from patients suffering from periodontitis. Fluorescence in situ hybridization represents a useful method for assessing the microbial ecology of the periodontal flora. |
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| Keywords: | Periodontitis Porphyromonas gingivalis Bacteroides forsythus In situ hybridization 16S rRNA Microbial ecology |
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