<Emphasis Type="Italic">Neochloris oleabundans</Emphasis> UTEX #1185: a suitable renewable lipid source for biofuel production |
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Authors: | Luísa Gouveia Ana Evangelista Marques Teresa Lopes da Silva Alberto Reis |
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Institution: | 1.Instituto Nacional de Engenharia e Tecnologia Industrial, INETI-DER, UB,Lisbon,Portugal;2.Instituto Nacional de Engenharia e Tecnologia Industrial, INETI-DB, UBB,Lisbon,Portugal |
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Abstract: | Energy crises, global warming, and climatic changes call for technological and commercial advances in manufacturing high-quality
transportation fuels from unconventional feedstocks. Microalgae is one of the most promising sources of biofuels due to the
high yields attained per unit area and because it does not displace food crops. Neochloris oleabundans (Neo) microalga is an important promising microbial source of single-cell oil (SCO). Different experimental growth and lipid
production conditions were evaluated and compared by using optical density (540 nm), dry-weight determination, and flow cytometry
(FC). Best Neo average biomass productivity was obtained at 30°C under conditions of nitrogen-sufficiency and CO2 supplementation (N+/30°C/CO2), with an average doubling time of 1.4 days. The second and third highest productivities occurred with N-sufficient cultures
without CO2 supplementation at 26°C (N+/26°C) and at 30°C (N+/30°C), with doubling times of 1.7 and 2.2 days, respectively. Microbial
lipid production was monitored by flow cytometry using Nile red (NR), a lipophilic fluorochrome that possesses several advantageous
characteristics for in situ screening near real time (at line). Results showed maximum lipid content (56%) after 6 days of
nitrogen depletion under nitrogen starvation without CO2 supplementation (N−/30°C), followed by N−/30°C/CO2 and N−/26°C conditions with 52% lipid content, after 5 and 6 days of N starvation, respectively. The adequate fatty acid
profile and iodine value of Neo lipids reinforced this microalga as a good source of SCO, in particular for use as biodiesel. |
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Keywords: | Neochloris oleabundans Lipid Oil Fatty acid Biodiesel Flow cytometry Nitrogen starvation |
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