Mitochondrial Permeability Transition Induced by Different Concentrations of Zinc |
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| Authors: | Xiao-Rong Liu Jia-Han Li Yue Zhang Yu-Shu Ge Fang-Fang Tian Jie Dai Feng-Lei Jiang Yi Liu |
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| Affiliation: | (1) State Key Laboratory of Virology, Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China;(2) Department of Chemistry and Life Sciences, Xianning University, Xianning, 437005, People’s Republic of China; |
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| Abstract: | Zinc is one of the required trace elements in animals, and it serves an important role in biological systems. However, high
levels of zinc are poisonous to organisms. So far, there exist conflicting reports about zinc ions-induced mitochondrial permeability
transition (MPT). We analyzed the effects of Zn2+ on MPT by monitoring mitochondrial swelling with the ultraviolet–visible light absorption spectrum, characterizing the fluidity
of the membrane with fluorescence anisotropy, detecting the transmembrane potential (Δψ) with fluorescence intensity, and
observing mitochondrial ultrastructure with transmission electron microscopy. Data reveal that low concentrations of zinc
ions can trigger MPT while high levels of zinc ions cannot, which implies that zinc ions’ toxicity cannot be the result of
a single simple mechanism. |
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