<Emphasis Type="Italic">Bacillus rhizosphaerae</Emphasis> sp. nov., an novel diazotrophic bacterium isolated from sugarcane rhizosphere soil |
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Authors: | Munusamy Madhaiyan Selvaraj Poonguzhali Jung-Sook Lee Keun-Chul Lee Kuppusamy Hari |
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Institution: | (1) Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India;(2) Biomaterials and Biocatalysts, Temasek Life Sciences Laboratory, National University of Singapore, Singapore, 117604, Singapore;(3) Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 111 Gwahangno, Yusong-gu, Daejeon, 305-806, Republic of Korea;(4) Division of Crop Production, Sugarcane Breeding Institute, ICAR, Coimbatore, 641007, Tamil Nadu, India |
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Abstract: | A Gram-positive, non-pigmented, rod-shaped, diazotrophic bacterial strain, designated SC-N012T, was isolated from rhizosphere soil of sugarcane and was subjected to a polyphasic taxonomic study. The strain exhibited
phenotypic properties that included chemotaxonomic characteristics consistent with its classification in the genus Bacillus. Sequence analysis of the 16S rRNA gene of SC-N012T revealed the closest match (98.9% pair wise similarity) with Bacillus clausii DSM 8716T. However, DNA–DNA hybridization experiments indicated low levels of genomic relatedness (32%) with this strain. The major
components of the fatty acid profile are iso-C15:0, anteiso-C15:0, iso-C17:0 and anteiso-C17:0. The diagnostic cell-wall diamino acid was meso-diaminopimelic acid. The G+C content of the genomic DNA is 43.0 mol%. The lipids present in strain SC-N012T are diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol and two unknown phospholipids. Their predominant
respiratory quinone was MK-7. Studies of DNA-DNA relatedness, morphological, physiological and chemotaxonomic analyses and
phylogenetic data based on 16S rRNA gene sequencing allowed strain SC-N012T to be described as members of novel species of the genus Bacillus, for which the name Bacillus rhizosphaerae sp. nov. is proposed. The type strain is SC-N012T (=DSM 21911T = NCCB 100267T). |
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