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Improved isolation of glucuronan from algae and the production of glucuronic acid oligosaccharides using a glucuronan lyase |
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| Authors: | Elboutachfaiti Redouan Delattre Cedric Petit Emmanuel El Gadda Mohamed Courtois Bernard Michaud Philippe El Modafar Cherkaoui Courtois Josiane |
| Affiliation: | a LPMV, Université de Picardie Jules Verne, IUT-GB Amiens, Avenue des facultés, Le Bailly, F-80025 Amiens Cedex 1, France b LGCB, Université Blaise Pascal-Polytech Clermont Ferrand, 24 rue des Landais, F-63174 Aubière, France c GREENTECH, Biopôle Clermont Limagne 63360 Saint Beauzire, France d LBVPA, Université Cadi Ayyad, Faculté des Sciences et Techniques Guéliz B.P. 549, Avenue Abdelkarim El Khattabi 40 000 Marrakech, Maroc |
| Abstract: | A new source for the production of bioactive glucuronic acid oligosaccharides (GlcUAOs) from the depolymerization of green seaweed Ulva lactuca glucuronan (Algal glucuronan) has been investigated. Algal glucuronan purification was optimized by the acidic precipitation method which allowed us to separate the polysaccharide mixture extracted from the cell wall of Ulva lactuca using hot water containing sodium oxalate. A series of the GlcUAOs were obtained by enzyme degradation of algal glucuronan with a glucuronan lyase (GL) isolated from Trichoderma strain. The putative bioactive GlcUAOs generated were then purified by size-exclusion chromatography in gram quantity and characterized by 1H/13C NMR spectroscopy and ESI-Q/TOF-mass spectrometry. |
| Keywords: | Glucuronan Green seaweed Oligosaccharides Polysaccharide lyase Glucuronic acid oligosaccharides Ulva lactuca |
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