Hybridization in Capparis spinosa L.: Molecular and morphological evidence from a Mediterranean island complex |
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| Institution: | 1. Istituto di Bioscienze e BioRisorse (IBBR) CNR, UOS di Palermo, Corso Calatafimi 414, 90129 Palermo, Italy;2. Dipartimento di Scienze Agrarie e Forestali, Università di Palermo, Via Archirafi 38, 90123 Palermo, Italy;1. Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan;2. Department of Biological Environment, Akita Prefectural University, Shimoshinjo-Nakano, Akita 010-0195, Japan;1. Laboratório de Biologia da Reprodução, Departamento de Botânica, Museu Nacional, Universidade Federal do Rio de Janeiro, Quinta da Boa Vista, Bairro São Cristovão, Rio de Janeiro/RJ, CEP 20940-040, Brazil;2. Laboratório de Biologia Reprodutiva, Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa/MG, CEP 36570-000, Brazil;1. MTA-DE Biodiversity and Ecosystem Services Research Group, Egyetem tér 1, Debrecen H-4032, Hungary;2. Technische Universität Bergakademie Freiberg, Interdisciplinary Ecological Centre, Leipziger Str. 29, Freiberg D-09596, Germany;3. Vienna University of Technology, Department of Geodesy and Geoinformation, Gußhausstraße 27-29, Vienna A-1040, Austria;4. ATMOTERM S.A., ul. ?angowskiego 4, 45-031 Opole, Poland;5. University of Debrecen, Böszörményi út 138, Debrecen H-4032, Hungary;1. Dipartimento di Scienze della Vita, Università degli Studi di Trieste, Giorgieri 10, 34123 Trieste, Italy;2. Dipartimento di Biologia Ambientale, Università degli Studi “La Sapienza”, Roma, Italy;3. Dipartimento di Sanità Pubblica e Malattie Infettive, Università degli Studi “La Sapienza”, Roma, Italy |
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| Abstract: | Inter-Simple Sequence Repeat (ISSR) molecular markers and morphological analysis were used in order to characterize wild populations and cultivated forms of orphan crop species Capparis spinosa L. in a Mediterranean island complex. Nineteen wild populations belonging to two different subspecies, C. spinosa subsp. spinosa and subsp. rupestris, were sampled in different environments in Sicily and the surrounding islets Lampedusa, Pantelleria and Salina. Different biotypes cultivated in Pantelleria and Salina were analysed. Six ISSR primers were selected for genetic characterization, and all clear and reproducible bands were scored and analysed. Among the 47 ISSR bands obtained, 97.5% were polymorphic. Results of AMOVA and STRUCTURE analysis suggested a clear genetic distinctness between subspecies at the regional level and suggested the existence of two taxonomic groups among wild populations, with different ecological preferences and distinctive morphological characters. Cultivated forms showed genetic affinity to subsp. rupestris. ISSR analysis not only provided specific molecular markers to discriminate the taxa, but also proved useful in supporting the hypothesis of a hybrid origin of the intermediate phenotypes found in overlapping distribution areas. The identified molecular markers provided a basic tool for the DNA fingerprinting of wild and commercial capers in the Mediterranean region and nearby territory. |
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| Keywords: | Commercial capers DNA fingerprinting Hybrids Intermediate phenotypes Ecological speciation ISSR markers |
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