| 支气管哮喘伴发抑郁大鼠模型的建立 |
| |
| 引用本文： | 孙宏伟,李承德,毛淑梅,宋玉萍,王艳郁.支气管哮喘伴发抑郁大鼠模型的建立[J].中国实验动物学报,2010,18(2):156-160,I0011. |
| |
| 作者姓名： | 孙宏伟 李承德 毛淑梅 宋玉萍 王艳郁 |
| |
| 作者单位： | 1. 潍坊医学院心理学教研室,潍坊,261053
2. 潍坊医学院药理学教研室,潍坊,261053 |
| |
| 基金项目： | 山东省教育厅科技计划项目，山东省自然科学基金资助项目 |
| |
| 摘 要： | 目的探讨建立支气管哮喘伴发抑郁动物模型的方法。方法选择Wistar大鼠,随机分为对照组与模型组,模型组采用卵蛋白(ovalbumin,OVA)激发法建立哮喘模型,在此基础上给予慢性轻度不可预见性应激(chronic unpredictable mild stress,CUMS)28d,观察大鼠体质量、体征、肺组织结构、支气管肺泡灌洗液白细胞计数等变化,并用Open-field实验评价大鼠活动度和好奇心,通过糖水消耗实验评价大鼠快感缺乏与否。结果与对照组相比,模型组大鼠体质量增长明显缓慢(P0.05);肺组织呈哮喘样病理改变;支气管肺泡灌洗液白细胞总数、嗜酸粒细胞及淋巴细胞增多;Open-field实验,大鼠垂直活动得分、水平活动得分显著降低(P0.05);糖水消耗量明显减少(P0.05)。结论OVA激发复合CUMS可成功制备支气管哮喘伴发抑郁大鼠模型。
|
| 关 键 词： | 支气管哮喘 抑郁 模型 慢性轻度不可预见性应激 大鼠 |
Establishment of a Rat Model of Bronchial Asthma Combined with Depression |
| |
| SUN Hong-wei,LI Cheng-de,MAO Shu-mei,SONG Yu-ping,WANG Yan-yu.Establishment of a Rat Model of Bronchial Asthma Combined with Depression[J].Acta Laboratorium Animalis Scientia Sinica,2010,18(2):156-160,I0011. |
| |
| Authors: | SUN Hong-wei LI Cheng-de MAO Shu-mei SONG Yu-ping WANG Yan-yu |
| |
| Institution: | SUN Hong-wei1,LI Cheng-de2,MAO Shu-mei2,SONG Yu-ping1,WANG Yan-yu1(1.Department of Applied Psychology,2.Department of Pharmacology,Weifang Medical College,Weifang 261053,China) |
| |
| Abstract: | Objective To establish a rat model of bronchial asthma combined with depression.Methods Twenty-two Wistar rats were randomly divided into control group and model group.The latter were sensitized with ovalbumin for 4 weeks and followed by CUMS for 4 weeks.Pathological changes of the lung tissue were observed by light microscopy and transmission electron microscopy.Eosinophil count and other inflammatory cell count in bronchoalveolar lavage fluid(BALF)were performed.The changes of behavior were detected by op... |
| |
| Keywords: | Bronchial asthma Depression Model Chronic unpredictable mild stress(CUMS) Rat |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
