Symbiotic and non-symbiotic expression of cgMT1, a metallothionein-like gene from the actinorhizal tree Casuarina glauca |
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| Authors: | Laplaze Laurent Gherbi Hassen Duhoux Emile Pawlowski Katharina Auguy Florence Guermache Fathia Franche Claudine Bogusz Didier |
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| Affiliation: | (1) Department of Plant Sciences, University of Cambridge, Cambridge, CB2 3EA, UK;(2) UMR Amélioration et Santé des Plantes (ASP), Université B. Pascal/INRA 24, avenue des Landais, 63177 Aubière Cedex, France;(3) IRD, UR116, 911 Avenue Agropolis, 34032 Montpellier Cedex 1, France;(4) Albrecht-von-Haller-Institut für Pflanzenwissenschaften, Biochemie der Pflanze, Universität Göttingen, Untere Karspüle 2, 37073 Göttingen, Germany |
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| Abstract: | A clone for a type 1 metallothionein (cgMT1) was isolated from a Casuarina glauca nodule cDNA library. The corresponding gene belongs to a small family and is highly expressed in roots and nitrogen-fixing nodules, whereas low expression was observed in aerial parts of the plant. The promoter region of cgMT1 was isolated and fused to the beta-glucuronidase (gus) gene. Transgenic Casuarinaceae plants showed that the cgMT1 promoter was most active in roots and in the oldest region of the shoot. In situ hybridization indicated that in nodules cgMT1 transcript is present in mature Frankia-infected cells and in the pericycle. Possible roles for cgMT1 in symbiotic and nonsymbiotic tissues are discussed. |
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| Keywords: | actinorhiza,Casuarina glauca,  -glucuronidase gene metallothionein symbiosis transgenic plant |
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-glucuronidase gene metallothionein symbiosis transgenic plant