Quantitative analysis of the redox states of cytochromes in a living L929 (NCTC) cell by resonance Raman microspectroscopy |
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| Authors: | Minoru Kakita Masanari Okuno Hiro‐o Hamaguchi |
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| Institution: | 1. Department of Chemistry, School of Science, The University of Tokyo, Hongo 7‐3‐1, Bunkyo, Toyo 113‐0033, Japan;2. Institute of Molecular Science and Department of Applied Chemistry, National Chiao Tung University, 1001 Ta Hsueh Road, Hsinchu 300, Taiwan |
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| Abstract: | Raman spectra and images of a living L929 (NCTC) cell have been measured with 532 nm excitation. Both reduced and oxidized forms of cytochromes b and c (cyt b and cyt c) have been observed in situ without any pretreatment. The redox states of cyts b and c have been assessed quantitatively with a spectral analysis. It has been found that reduced cyt c is more abundant than oxidized cyt c, while oxidized cyt b is slightly more abundant than reduced cyt b in a living cell. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) |
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| Keywords: | cytochrome redox quantitative analysis resonance Raman spectroscopy mitochondria |
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