Ultrastructural demonstration of NAD-pyrophosphorylase activity in mouse liver nuclei |
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| Authors: | Eberhard Unger Igor B. Buchwalow Manfred Hartmann Wladimir M. Kleschtschinow Nathan T. Raikhlin |
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| Affiliation: | (1) Central Institute of Microbiology and Experimental Therapy, Academy of Sciences, Jena, German Democratic Republic;(2) Institute of Experimental and Clinical Oncology, Academy of Medical Sciences, Moscow, USSR |
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| Abstract: | Summary Ultrastructural demonstration of NAD-pyrophosphorylase activity (E.C. 2.7.7.1) in isolated mouse liver nuclei was investigated with the use of an electronhistochemical procedure based on the precipitation of pyrophosphate ions with lead ions under conditions permitting simultaneous ATPase inhibition by formaldehyde/ethanol prefixation. In isolated mouse liver nuclei activity of NAD-pyrophosphorylase was found in nucleoli, in interchromatin granules, coiled bodies and strand-like structures in nucleoplasm. |
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