Kinetics of albumin- and alpha-fetoprotein-production during rat liver development |
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Authors: | Abderrahim Elmaouhoub József Dudas Giuliano Ramadori |
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Institution: | (1) Department of Internal Medicine, Section of Gastroenterology and Endocrinology, Georg-August-University, Goettingen, Germany;(2) Department of Gastroenterology and Endocrinology, Georg-August-University, Robert-Koch-Strasse 40, Goettingen, 37075, Germany |
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Abstract: | Synthesis of most of the plasma proteins is one of the main functions of the hepatocytes. Albumin synthesis is quantitatively
the most abundant. In the present study we investigated albumin- and alpha-fetoprotein-gene-expression, and the function of
the secretory apparatus during rat liver development. To this purpose we used the method of radioactive biosynthetic labeling
of newly synthesized albumin and alpha-fetoprotein (AFP) to monitor the secretory capacity of endodermal cells derived from
ventral foregut region (embryonic day 10, E10), and of embryonic and fetal hepatoblasts. Synthesis and secretion of albumin
and AFP were already detected in the low numbered ventral foregut endodermal cells; fibrinogen synthesis was detectable in
the E12 hepatoblasts, which were in higher number. The whole secretory machinery was functional from the earliest stages of
liver development, and the speed of secretion was comparable with that of the adult hepatocytes. There was almost 4-fold increase
of hepatoblasts cell volume in fetal stage compared with embryonic stage. The model used suggests that the hepatocyte secretory
apparatus is already functional before the emergence of the liver bud. This is the first comparative report to analyze the
hepatocyte secretory function, cell proliferation and cell volume during liver development. |
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Keywords: | Secretory apparatus Albumin Differentiation Cell volume Proprotein convertases Subtilisin-like proteinases |
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